5 ML PROTEIN FREE FILTRATE.
ADD 1 PART WHOLE BLOOD TO 2 PARTS COLD 8%* PERCHLORIC ACID.
LET STAND IN REFRIGERATOR FOR 5-10 MINUTES TO INSURE PRECIPITATION OF PROTEIN.
CENTRIFUGE OR FILTER AND SUBMIT CLEAR FILTRATE.
* MADE BY DILUTING 11.4 ML OF 70% PERCHLORIC ACID TO 100 ML WITH DISTILLED H20.